Journal: bioRxiv
Article Title: Shifts in protein aggregate stability define proteostasis decline in the aging human brain
doi: 10.64898/2026.03.27.714902
Figure Lengend Snippet: A. Western blot of SDS insoluble PIN1, 6 young and 6 old. B . Western blot of SDS insoluble CST3, 6 young and 6 old. C . Western blot of Sarkosyl insoluble MAPT (Tau), 4 young and 4 geriatric. D . Western blot of sarkosyl insoluble HTT, 4 young and 4 geriatric. For all blots each lane represents an independent biological sample (individual donor).
Article Snippet: Membranes were blocked in 5% non-fat milk in TBS-T for 1 hour at room temperature, then incubated overnight at 4°C on a rocker with primary antibodies against PIN1 (mouse monoclonal, 1:2000, Cat. No. 68127-1-Ig, Proteintech), Cystatin C (CST3 rabbit polyclonal, 1:6000, Cat. No. 12245-1-AP, Proteintech), total tau (Tau5, mouse monoclonal, 1:500, Cat. No. AHB0042, Invitrogen), and HTT (rabbit polyclonal, N-terminal, 1:150, Cat. No. H7540, Sigma-Aldrich).
Techniques: Western Blot